Team Philippe RONDARD
Neuroreceptors, dynamics and functions
Project Methods and tools for studying mGlu and GABA-B receptors in vitro and in their native environment
PRINCIPAL INVESTIGATOR
IGF staff involved
Clément PEYRON
PhD student Revvity
Junke LIU
Post-doc CNRS
Cyrielle VIAL
IECN CNRS
It is important to develop new methods and tools to study GPCRs in their native environment (G protein coupling, signaling, oligomerization, desensitization and recycling) to better understand their function and develop new drug candidates. We are developing biosensors based on Förster-type resonance energy transfer (FRET, HTRFÒ) or bioluminescence (BRET) technologies for the study of all GPCRs. On the other hand, we are developing specific ligands to study mGlu and GABA-B receptors, such as small molecules or camelid antibodies (nanobodies), and some of them are light-controllable.
FRET technologies as methods of choice to study cell surface GPCRs.
Main publications
• Xu C., et al. (2024) Nat Comm, 15, 1990.
• Meng J., et al. (2024) Nat Chem Biol, 18, 894-903.
• Lecat-Guillet N., et al. (2023) Sci Adv, 9: eadf1378.
• Liu J., et al. (2022) Nat Comm 13, 6365.
• Haubrich J., et al. (2021) PNAS 118, e2105848118.
• Cao A.M., et al. (2021) Nat Comm 12:5426.
• Liu H., et al. (2020) PNAS 117, 21711-21722.
• Scholler P., et al. (2017) Nat Chem Biol 13, 372-380.
• Lecat-Guillet N., et al. (2017) Cell Chem Biol 24, 360-370.
• Olofsson L., et al. (2014) Nat Comm 5:5206.
• Doumazane E., et al. (2013) PNAS 110, E5742-5743.
• Doumazane E., et al. (2011) FASEB J 25, 66-77.
Funding
• 2024-2028 ANR 2023-NANOMGLU – Partner
• 2019-2023 ANR 2018-DYNAMUR2 – Coordinator
• 2014-2018 ANR 2013-RSIGNAL – Coordinator
• 2014-2017 ANR 2013-NANOGLUR – Coordinator
• 2011-2014 ANR 2009-GLUSENSE – Partner
Collaborations
• Revvity Cisbio (Codolet, France)
• Isabelle McCort & Francine Acher (Université Paris-Cité, Paris, France)
• Amadeu Llebaria (University of Barcelona, Barcelona, Spain)
Alumni
• Alexandre Bouyssou (Ph.D student, 2019-2023)
• Jordi Haubrich (Ph.D student, 2017-2021)
• Mélanie Da Silva (Ph.D student, 2013-2017)
• Fanny Dubois (Postdoc, 2013-2016)
• Pauline Scholler (Ph.D student, 2009-2013)
• Etienne Doumazane (Ph.D student, 2007-2011)
Patents
• Trinquet E, Dupuis E, Bdioui S, Rondard P, Pin J-P, Roux T WO2020/157441, 2019
• Pin J-P, Rondard P, Soulé J, Da Silva M, Dupuis E, Roux T, Trinquet E, Mailhac C, Chames P, Baty D WO2020/021213, 2018
• Pin J-P, Rondard P, Soulé J, Da Silva M, Roux T, Dupuis E, Trinquet E WO2019/020964A1, 2017
• Pin J-P, Baty D, Mathis G, Nevoltris D, Chames P, Scholler P, Rondard P PCT/EP2015/065228 – EP3164415A3164411 – WO2016001417A, 2015
• Comps-Agrar L, Pin J, Brock C PTC/FR2011/052812 PTC/FR2011/052812, 2010
• Acher F, Selvam C, Pin J-P PCT/IB2010/051200, WO 2010/106526A1, 2010
• Trinquet E, Zwier J, Mathis G, Pin J-P, Durroux T FR 09 55667, PCT/FR2010/051700, WO 2011/018586 A2, 2009
• Doumazane E, Pin J-P, Zwier J, Trinquet E FR 09 52908, PTC/FR2010/050814, WO 2010/125314 A1, 2009
