Team Julie PERROY
Pathophysiology of synaptic transmission
Project Integration of neuronal signalling
PRINCIPAL INVESTIGATOR
IGF staff involved
All team staff
Neuronal activity and neuromodulatory context continuously adjust the efficacy of synaptic transmission. The calcium signal which integrates neuronal activity and glutamatergic inputs is one major trigger of plasticity events. The context is integrated through various other intracellular signals (kinases, cyclic nucleotides…) We combine biosensor imaging and biophysical modeling to explore how these signaling pathways are interacting functionally.
Calcium and cAMP are critical determinants of synaptic plasticity. These two signaling pathways interact through various molecular mechanisms, and how these signals interact underlies plasticity events in learning and memory. We analyze how such interactions occur and how they are organized in time and space using biosensor imaging in hippocampal or cortical neurons, during the course of protocols known to induce plasticity.
Various genetically-encoded biosensors report, with a good spatial and temporal resolution, the intracellular concentration in cAMP, cGMP, calcium, the activation of PKA, CREB… in pyramidal neurons in brain slice preparations. Here, the cAMP biosensor Epac-SH150 shows a transient increase in cAMP (arrow) after the photorelease of NMDA.
Main publications
• Salgado-Almario et al. (2022) Theranostics 12, 1012.
• Mota et al. (2021) Br J Pharmacol 178, 4873.
• Vincent et al. (2021) J Neurosci Methods 109305.
• Stoufflet et al. (2020) Sci Adv 6, eaba3992.
• Lupieri et al. (2020) J Cell Sci 133, jcs245969.
• Nair et al. (2019) Neuropharmacology 146, 74.
• Stoufflet et al. (2019) BioRxiv
• Betolngar et al. (2019) Cereb Cortex 29, 5022.
Funding
• 2022-2026 ANR LEARN – Partner
Collaborations
• Juan Llopis (Albacete, Espagne)
• Oliver Griesbeck (Munich)
Alumni
• Liliana Castro (Enseignant-chercheur)
• Ségolène Bompierre (thèse)
• Elia Mota (thèse)
