Team Chris JOPLING
Cardiac Development, Disease and Regeneration
Project Cellular interactions driving cardiac remodeling
PRINCIPAL INVESTIGATOR
IGF staff involved
Chloé COMBE
IE INSERM (CDD)
Complex interactions between cardiac cells regulate development and disease progression. We use multiple animal and in vitro models to study the genes and cellular mechanisms at play. We are particularly interested in how cardiac fibroblasts, the main cells responsible for extracellular matrix deposition, could be manipulated to alleviate heart failure.
Fibroblasts in cardiac remodeling
Fibroblasts in cardiac remodeling
Cardiac fibroblasts are the main cell-type responsible for the production of extracellular matrix in the heart. We use mouse, zebrafish and iPS models to explore their functions in heart failure and regeneration. Regulating cardiac fibroblast activity is a major clinical issue.
Single cell UMAP plot from zebrafish heart.
Main publications
• Rolland L, Harrington A, et al. (2023) J Mol Cell Biol., Mar 29;14(10):mjac059.
Funding
• 2019-2024 ANR-18-ECVD-0006 – Coordinator
Collaborations
• Paola Cattaneo, IRCCS Monzino Cardiology Center, Milan.
• Justus Stenzig, University Medical Center Hamburg-Eppendorf.
• Luis Luna Zurita, CNIC, Madrid.
• Pierre Sicard, PhyMedExp, Montpellier.
Alumni
• Alenca Harrington (Thèse, 2019-2023)
The zebrafish as a model for ARVD
ARVD (Arrhythmogenic right ventricular dysplasia) is a rare cardiac pathology and the underlying mechanisms are still poorly understood. In collaboration with other teams, we are using the zebrafish model to characterize the cardiac function of genes potentially associated with this pathology.
Zebrafish larvae.
Funding
• 2023-2027 ANR-23-CE14-0008– Partner.
Collaborations
• Vincent Probst, l’institut du thorax, Nantes.
• Estelle GANDJBAKHCH, Sorbonne Université, Paris.
• Angelo TORRENTE, IGF, Montpellier.
Remodeling of the pituatry
Endocrinopathies induce dramatic functional changes in the endocrine cells of the pituatry. This project involves characterizing these changes, notably by single-nuclei based analysis. Furthermore, we are studying the tissue structural changes/remodeling that accompany the euthyroid and hypothyroid states.
Single nuclei RNA-seq analysis of pituatry hypothyroidism.
Funding
• 2022-2025 ANR-22-CE14-0001 – Partner.
Collaborations
• Patrice Mollard, IGF, Montpellier.
