Team Hugues DUFFAU – Jean-Philippe HUGNOT
Brain plasticity, stem cells and diffuse low-grade gliomas
Project Spatial heterogeneity of low-grade gliomas
PRINCIPAL INVESTIGATOR
Diffuse low-grade IDH-mutated gliomas are tumours that inevitably progress to a higher grade and lead to the patient’s death. These tumours are heterogeneous both histologically and molecularly, and contingents of different grades may be observed within the same tumour, reflecting this lesion continuum. In some grade 2 gliomas, we have described the presence of early foci of transformation (2+), without sufficient criteria for grade 3, and with a prognosis intermediate between grade 2 and 3.
We hope to gain a better understanding of the molecular mechanisms underlying this evolution at transcriptional and protein level.
Tumour progression in low-grade gliomas
A single-cell study of the expression of phenotypic, molecular, metabolic and epigenetic markers in different areas of these tumours, using a spatial proteomics technique based on mass cytometry.
Pedeutour et al., 2014.
Histopathological characteristics of grade 2 gliomas containing hypercellular foci (G2+)
a,b: hypercellular foci.
c,d: hypercellularity, increased number of vessels and increased atypia within the focus (c) in contrast to the rest of the tumour (d).
e,f: presence within a tumour of a single vessel with microvascular proliferation (e), while no other vascular abnormality is observed in the rest of the tumour (f).
Study of microenvironment/tumour relationships
Images of perineuronal satellitosis and perivascular lymphoid cuffing
Main publications
• Leventoux N, et al (2020) Sci Rep.10(1):5504.
• Darlix A, el al, (2020) Neurology. 2020 94(8)
• Pedeutour-Braccini Z et al, (2015) Virchows Arch. 466(4):433-44.
Funding
• 2017 PRTK, GSO Emergence
Collaborations
• Lucille Stuani, IRCM, Montpellier
• Monika Hegi, University of Lausanne, Switzerland
Alumni
• N. Leventoux, PhD (2018-2019)
• Z. Peudeutour, Master (2014)
Patents
• Hugnot, JP, Guichet PO, Teigell M, Bieche Y, Lidereau R, Joubert D, Bauchet L, Rigau V. European Patent 10305805.3, PCT/EP2011/062578
