MGX plateform
MGX – Montpellier GenomiX
The MGX-Montpellier GenomiX platform’s IGF platform offers the scientific community and biotech companies a wide range of genomics techniques based on very high throughput sequencing (NGS). It is open to Montpellier, French and foreign laboratories in the fields of biology-health, agronomy, environment and biodiversity-evolution.
High-throughput DNA sequencing or NGS is used for a wide range of applications: whole genome sequencing, variant identification, genotyping, epigenetics, chromatin architecture, transcriptomics, small RNA transcriptomics, CRISPR-Cas9 screens…
Some of these applications can be carried out on single cells, in suspension or on organ sections, in particular transcriptomics and chromatin accessibility.
The platform also offers statistical analysis of NGS data for many of the above applications.
The platform employs 11 people and in 2023 carried out 124 projects for 80 teams from 49 laboratories and one private company. It is equipped with Illumina (NovaSeq, MiniSeq) and Oxford Nanopore Technologies (MinION) sequencers, robots for library preparation and quantification (2 mosquito HV robots and 2 Freedom EVO robots), and a range of sample and library quality control equipment (Fragment Analyzer, Qbit, PCR machines, etc.). It also has a microfluidic device for single-cell transcriptomics (Chromium 10X Genomics). It is currently setting up NGS-based spatial transcriptomics techniques.
The facility is ISO9001 and NFX50-900 certified. It is accredited by IBiSA (www.ibisa.net) and is part of the central core of the France Génomique national infrastructure (www.france-genomique.org). More information here: https://www.mgx.cnrs.fr/.
IGF Sud 022
04 34 35 92 40
IGF Sud 021 04 34 35 92 41
IGF Sud 021 04 34 35 92 41
IGF Sud 020 04 34 35 92 39
IGF Sud 020 04 34 35 92 39
IGF Sud 021 04 34 35 92 61
IGF Sud 020 04 34 35 92 39
IGF Sud 022 04 34 35 92 40
IGF Sud 020 04 34 35 92 39
IGF Sud 021 04 34 35 92 41
IGF Sud 020 04 34 35 92 39IGF Publications
- Salles J, Eddiry S, Amri S, Galindo M, Lacassagne E, George S, Mialhe X, Lhuillier É, Franchitto N, Jeanneteau F, Gennero I, Salles JP, Tauber M. Differential DNA methylation in iPSC-derived dopaminergic neurons: a step forward on the role of SNORD116 microdeletion in the pathophysiology of addictive behavior in Prader-Willi syndrome. Mol Psychiatry. 2024 Apr 2. doi: 10.1038/s41380-024-02542-4.
- Puighermanal E, Castell L, Esteve-Codina A, Melser S, Kaganovsky K, Zussy C, Boubaker-Vitre J, Gut M, Rialle S, Kellendonk C, Sanz E, Quintana A, Marsicano G, Martin M, Rubinstein M, Girault JA, Ding JB, Valjent E. Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum. Nat Commun. 11:1957. doi: 10.1038/s41467-020-15716-9.
- Reynès C, Kister G, Rohmer M, Bouschet T, Varrault A, Dubois E, Rialle S, Journot L, Sabatier R. ISoLDE: a data-driven statistical method for the inference of allelic imbalance in datasets with reciprocal crosses. Bioinformatics. 2020, 36:504-513 doi: 10.1093/bioinformatics/btz564
- Baudement MO, Cournac A, Court F, Seveno M, Parrinello H, Reynes C, Sabatier R, Bouschet T, Yi Z, Sallis S, Tancelin M, Rebouissou C, Cathala G, Lesne A, Mozziconacci J, Journot L, Forné T. High-salt-recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodies. Genome Res. 2020, 28:1733-46
Publications external to IGF
- Leiba J, Sipka T, Begon-Pescia C, Bernardello M, Tairi S, Bossi L, Gonzalez AA, Mialhe X, Gualda E, Loza-Alvarez P, Blanc-Potard A, Lutfalla G, Nguyen-Chi ME (2024) Dynamics of macrophage polarization support Salmonella persistence in a whole living organism. Elife. 13:e89828. doi: 10.7554/eLife.89828. PMID: 38224094.
- Kuhl H, Euclide PT, Klopp C, Cabau C, Zahm M, Lopez-Roques C, Iampietro C, Kuchly C, Donnadieu C, Feron R, Parrinello H, Poncet C, Jaffrelo L, Confolent C, Wen M, Herpin A, Jouanno E, Bestin A, Haffray P, Morvezen R, de Almeida TR, Lecocq T, Schaerlinger B, Chardard D, Żarski D, Larson WA, Postlethwait JH, Timirkhanov S, Kloas W, Wuertz S, Stöck M, Guiguen Y. Multi-genome comparisons reveal gain-and-loss evolution of anti-Mullerian hormone receptor type 2 as a candidate master sex-determining gene in Percidae. BMC Bio 2024 Jun 26;22(1):141. doi: 10.1186/s12915-024-01935-9. PMID: 38926709
- Esnault C, Magat T, Zine El Aabidine A, Garcia-Oliver E, Cucchiarini A, Bouchouika S, Lleres D, Goerke L, Luo Y, Verga D, Lacroix L, Feil R, Spicuglia S, Mergny JL, Andrau JC (2023) G4access identifies G-quadruplexes and their associations with open chromatin and imprinting control regions. Nat Genet. 55:1359-69. doi: 10.1038/s41588-023-01437-4.
- Boulanger M, Aqrouq M, Tempé D, Kifagi C, Ristic M, Akl D, Hallal R, Carusi A, Gabellier L, de Toledo M, Sigurdsson J-O, Kaoma T, Andrieu-Soler C, Forné T, Soler E, Hicheri Y, Gueret E, Vallar L, Olsen JV, Cartron G, Piechaczyk M, Bossis G. DeSUMOylation of chromatin-bound proteins limits the rapid transcriptional reprogramming induced by daunorubicin in acute myeloid leukemias. Nucleic Acids Res. 2023, 51:8413-33. doi: 10.1093/nar/gkad581.
- Jay P, Chouteau M, Whibley A, Bastide H, Parrinello H, Llaurens V, Joron M. Mutation load at a mimicry supergene sheds new light on the evolution of inversion polymorphisms. Nat Genet. 2021, 53:288-293 doi: 10.1038/s41588-020-00771-1.
- Du K, Stöck M, Kneitz S, Klopp C, Woltering JM, Adolfi MC, Feron R, Prokopov D, Makunin A, Kichigin I, Schmidt C, Fischer P, Kuhl H, Wuertz S, Gessner J, Kloas W, Cabau C, Iampietro C, Parrinello H, Tomlinson C, Journot L, Postlethwait JH, Braasch I, Trifonov V, Warren WC, Meyer A, Guiguen Y, Schartl M. The sterlet sturgeon genome sequence and the mechanisms of segmental rediploidization. Nat Ecol Evol. 2020 Jun;4(6):841-852. doi: 10.1038/s41559-020-1166-x. PMID: 32231327
