IPAM plateform
Small Animal Imaging platform of Montpellier
Patrice
Mollard
DRCE, CNRS
Chrystel
Lafont
IR2, Inserm
IPAM is an intravital imaging platform integrating longitudinal and multimodal monitoring of the animal (rodents, fish, insects, invertebrates), where the latter is its own control, for the assessment of normal biological functions or those altered by pathology.
IPAM brings together 3 technical core facilities, offering complementary intravital imaging modalities that provide real-time anatomical, functional and molecular information from the subcellular scale to the whole organism.
IPAM gathers 3 technical core facilities: IPAM-IGF, IPAM-IRCM, IPAM-Phymedex.
Their main equipments/applications are: IPAM-IGF, single/multiphoton intravital cellular imaging coupled with optical manipulations (optogenetics, ablation, etc.) targeted at the cellular level (microscopes and miniscopes); IPAM-Phymedex, anatomical, functional and haemodynamic characterisation using rapid high-resolution ultrasound/photo-acoustic imaging; and IPAM-IRCM, non-invasive monitoring of tumour progression and characterisation of potential new therapeutic molecules using nuclear imaging and bioluminescence.
Our medium-term goal is to continue to reduce the use of animals by offering in vivo/ex vivo imaging of reconstituted biological tissues such as organoids, PDX, or cell assemblies integrated into nano-chips.
IPAM is a member of BioCampus Montpellier, IBISA, the national infrastructures France-Bioimaging (IPAM-IGF) and France-Lifeimaging (IPAM-IRCM, IPAM-Phymedex), and its services/fee access are ISO-9001 certified and auditable by the CNRS.
Their main equipments/applications are: IPAM-IGF, single/multiphoton intravital cellular imaging coupled with optical manipulations (optogenetics, ablation, etc.) targeted at the cellular level (microscopes and miniscopes); IPAM-Phymedex, anatomical, functional and haemodynamic characterisation using rapid high-resolution ultrasound/photo-acoustic imaging; and IPAM-IRCM, non-invasive monitoring of tumour progression and characterisation of potential new therapeutic molecules using nuclear imaging and bioluminescence.
Our medium-term goal is to continue to reduce the use of animals by offering in vivo/ex vivo imaging of reconstituted biological tissues such as organoids, PDX, or cell assemblies integrated into nano-chips.
IPAM is a member of BioCampus Montpellier, IBISA, the national infrastructures France-Bioimaging (IPAM-IGF) and France-Lifeimaging (IPAM-IRCM, IPAM-Phymedex), and its services/fee access are ISO-9001 certified and auditable by the CNRS.
IPAM is a BioCampus plateform.
Longitudinal (1h, 3h and 24h) dorsal chamber imaging of breast cancer cells (green) following injection of a specific fluorescent antibody (magenta) [doi: 0.1016/j.jconrel.2019.11.014].
IGF (IPAM-IGF) Publications
- Romanò N, Lafont C, Campos P, Guillou A, Fiordelisio T, Hodson DJ,Mollard P, Schaeffer M. Median eminence blood flow influences food intake by regulating ghrelin access to the metabolic brain. JCI Insight. 2023 Feb 8;8(3):e165763. doi: 10.1172/jci.insight.165763. PMID: 36574295
- El Cheikh Hussein L, Fontanaud P,Mollard P, Bonnefont X. Nested calcium dynamics support daily cell unity and diversity in the suprachiasmatic nuclei of free-behaving mice. PNAS Nexus. 2022 Jul 11;1(3):pgac112. doi: 10.1093/pnasnexus/pgac112. eCollection 2022 Jul. PMID: 36741435
- Villar ME, Pavão-Delgado M, Amigo M, Jacob PF, Merabet N, Pinot A, Perry SA, Waddell S,Perisse E. Differential coding of absolute and relative aversive value in the Drosophila brain. Curr Biol. 2022 Nov 7;32(21):4576-4592.e5. doi: 10.1016/j.cub.2022.08.058. Epub 2022 Sep 13. PMID: 36103878
- Golan M, Boulanger-Weill J, Pinot A, Fontanaud P, Faucherre A, Gajbhiye DS, Hollander-Cohen L, Fiordelisio-Coll T, Martin AO,Mollard P. Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction. Sci Adv. 2021 Feb 19;7(8):eabc8475. doi: 10.1126/sciadv.abc8475. Print 2021 Feb.PMID: 33608269
- Cadilhac C, Bachy I, Forget A, Hodson DJ, Jahannault-Talignani C, Furley AJ, Ayrault O, Mollard P, Sotelo C,Ango F. Excitatory granule neuron precursors orchestrate laminar localization and differentiation of cerebellar inhibitory interneuron subtypes. Cell Rep. 2021 Mar 30;34(13):108904. doi: 10.1016/j.celrep.2021.108904. PMID: 33789110
- Hoa O,Lafont C, Fontanaud P, Guillou A, Kemkem Y, Kineman RD, Luque RM, Fiordelisio Coll T, Le Tissier P, Mollard P. Imaging and Manipulating Pituitary Function in the Awake Mouse. 2019 Oct 1;160(10):2271-2281. doi: 10.1210/en.2019-00297.PMID: 31329247
- Arango-Lievano M, Boussadia B, De Terdonck LDT, Gault C, Fontanaud P,Lafont C, Mollard P, Marchi N, Jeanneteau F. Topographic Reorganization of Cerebrovascular Mural Cells under Seizure Conditions. Cell Rep. 2018 Apr 24;23(4):1045-1059. doi: 10.1016/j.celrep.2018.03.110.PMID: 29694884
Publications external to IGF
- Odelin G,Faucherre A, Marchese D, Pinard A, Jaouadi H, Le Scouarnec S; FranceGenRef Consortium; Chiarelli R, Achouri Y, Faure E, Herbane M, Théron A, Avierinos JF, Jopling C, Collod-Béroud G, Rezsohazy R, Zaffran S. Variations in the poly-histidine repeat motif of HOXA1 contribute to bicuspid aortic valve in mouse and zebrafish. Nat Commun. 2023 Mar 20;14(1):1543. doi: 10.1038/s41467-023-37110-x.PMID: 36941270
- Espinosa-Carrasco G, Le Saout C, Fontanaud P, Stratmann T, Mollard P,Schaeffer M, Hernandez J. CD4+ T Helper Cells Play a Key Role in Maintaining Diabetogenic CD8+ T Cell Function in the Pancreas. Front Immunol. 2018 Jan 18;8:2001. doi: 10.3389/fimmu.2017.02001. eCollection 2017.PMID: 29403481
- Debie P, Lafont C, Defrise M, Hansen I, van Willigen DM, van Leeuwen FWB, Gijsbers R, D’Huyvetter M, Devoogdt N, Lahoutte T, Mollard P, Hernot S. Size and affinity kinetics of nanobodies influence targeting and penetration of solid tumours. J Control Release. 2020 Jan 10;317:34-42. doi: 10.1016/j.jconrel.2019.11.014. Epub 2019 Nov 14. PMID: 31734445
