ARPEGE platform

Facility, Pharmacology – Screening – Interactom

Laurent Prezeau
DR2, CNRS
Damien Maurel
IR1, Inserm

The ARPEGE platform is dedicated to extensive analysis of the structural and functional features of protein complexes. It allows the characterization of all kinds of specific interactions either between ligand and target, protein partners, and signaling complexes. The ARPEGE platform is managed by a team of highly experienced receptor biology and luminescence-based technique specialists. Its service offer is made available to academic laboratories, biotech as well as pharma and other industrial companies.

Our team, comprising one researcher and three engineers, has solid expertise in the use of luminescence techniques (fluorescence, FRET, TR-FRET, HTRF, BRET). The facility is equipped with 9 devices (medium throughput plate readers) and a liquid handling robot. The various cell-based assays available on the facility enable analysis of ligand-target binding, protein-protein interactions, activation of signaling pathways (G proteins, arrestins, GRK, internalization, etc.), second messenger production (cAMP, IP1, calcium, etc.) or phosphorylation of signaling proteins (ERK, Akt, etc.).

The platform offers its services on a fee-for-service basis to public laboratories and companies. We offer either open-access services (after appropriate training, customers have access to the equipment to carry out their project), or project/service agreements (platform engineers carry out all the experiments in accordance with the customer).

The ARPEGE facility is certified IBiSA and ISO:9001v2015 (since 2014).

More information on www.arpege.cnrs.fr

ARPEGE is a BioCampus plateform.

Screening of a small series of molecules on mu-opioid receptor signaling.

Researchers
Laurent
Prezeau
DR2, CNRS
  IGF Nord 221a
  04 34 35 93 07
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Technicians and engineers
Isabelle
Brabet
IEHC, CNRS
  IGF Nord 219
  04 34 35 93 13
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Damien
Maurel
IR1, Inserm
  IGF Nord 219
  04 34 35 93 13
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Claire
Vol
IEHC, CNRS
  IGF Nord 219
  04 34 35 93 13
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IGF Publications
  • Dumazer A, Gómez-Santacana X, Malhaire F, Jopling C, Maurel D, Lebon G, Llebaria A, Goudet C. Optical control of adenosine A2A receptor using istradefylline photosensitivity. ACS Chemical Neurosciences. 2024 doi.org/10.1021/acschemneuro.3c00721
  • Cong X, Maurel D, Déméné H, Vasiliauskaité-Brooks I, Hagelberger J, PeyssonF, Saint-Paul J, Golebiowski J, Granier S, Sounier R. Molecular insights into the biased signaling mechanism of the μ-opioid receptor. Molecular Cell. 2021 doi: 10.1016/j.molcel.2021.07.033
  • Healey RD, Saied EM, Cong X, Karsai G, Gabellier L, Saint Paul J, Del Nero E, Jeannot S, Drapeau M, Fontanel S, Maurel D, Basu S, Leyrat C, Golebiowski J, Bossis G, Bechara C, Hornemann T, Arenz C, Granier S. Discovery and mechanism of action of small molecule inhibitors of ceramidases. Angewandte Chemie. 2021 doi.org/10.1002/anie.202109967
  • Zindel D , Mensat P , Vol  C , Homayed  Z , Charrier-Savournin  F , Trinquet  E , Banères  JL, Pin  JP , Pannequin  J , Roux  T , Dupuis  E, Prézeau  G protein-coupled receptors can control the Hippo/YAP pathway through Gq signaling. FASEB J. 2021 doi: 10.1096/fj.202002159R.
Publications external to IGF
  • Kampen S, Rodríguez D, Jørgensen M, Kruszyk-Kujawa M, Huang X, Collins M, Boyle N, Maurel D, Rudling A, Lebon G, and Carlsson J. Structure-Based Discovery of Negative Allosteric Modulators of the 2 Metabotropic Glutamate Receptor 5. ACS Chem Biol. 2022 doi: 10.1021/acschembio.2c00234
  • Liu J, Hengmin Tang, Xu C, Zhou S, Zhu X, Li Y, Prézeau L, Xu T, Pin JP, Rondard P, Ji W, Liu J. Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor. Nat Commun. 2022; 13: 6365. doi: 10.1038/s41467-022-34056-4
  • Mills, N. Aissaoui, D. Maurel, J. Elezgaray, F. Morvan, J. J. Vasseur, E. Margeat, R. B. Quast, J. Lai Kee-Him, N. Saint, C. Benistant, A. Nord, F. Pedaci, G. Bellot. A modular spring-loaded actuator for mechanical activation of membrane proteins. Nature Com. 2022 doi: 10.1038/s41467-022-30745-2